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LIF

CAT: 0209-200-007Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0209-200-007Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.
Synonyms
LIF; CDF; DIA; HILDA; MLPLI
NCBI Gene ID
3976
UniProt
P15018
Accession Number
NP_002300.1
Accession Number mRNA
NM_002309.4
Chromosomal Location
22q12.2
Reactivity
Human
Cross Reactivity
Human
Sequence
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Assay Protocol
The lyophilized LIF should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Endotoxin
< 0.1 ng per µg (Ieu/µg) of rh LIF
Purity
> 98% by SDS-PAGE
Bioactivity
The ED50 as determined by the M1 cell differentiation assay is in the range of 0.1 - 0.5 ng/ml.
Length
180
Form
Lyophilized
Buffer
50mM acetic acid
Reconstitution
Water
Molecular Weight
19.7 kDa
Storage Conditions
Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid repeated freeze-thaw cycles.
Host or Source
E. coli
N Terminal Sequence
SPLPIT

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