Leptin

• Description:

  Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  25608

• UniProt:

  P50596

• Accession Number:

  NP_037208

• Accession Number mRNA:

  NM_013076

• Chromosomal Location:

  4q22

• Reactivity:

  Rat

• Cross Reactivity:

  Mouse, Rat

• Sequence:

  MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

• Length:

  147

• Form:

  Lyophilized

• Molecular Weight:

  16.2 kDa

• Host or Source:

  E. coli