IGF-BP3

• Description:

  Human IGF-BP3, also known as growth-hormone-dependant binding protein, is 28.9 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in alpha-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-2, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF and EGF. Recombinant human IGF-BP3 is a 28.8 kDa protein consisting of 264 amino acid residues.

• Synonyms:

  IGFBP3; IBP3; BP-53

• NCBI Gene ID:

  3486

• UniProt:

  P17936

• Accession Number:

  NP_001013416

• Accession Number mRNA:

  NM_001013398

• Chromosomal Location:

  7p13-p12

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is ≤ 0.2 µg/ml in the presence of 15 ng/ml of human IGF-II.

• Length:

  264

• Form:

  Lyophilized

• Molecular Weight:

  28.8 kDa

• Host or Source:

  E. coli