IGF-1

• Description:

  IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.

• Synonyms:

  Igf1; Igf-1; Igf-I; C730016P09Rik

• NCBI Gene ID:

  24482

• UniProt:

  P08025

• Accession Number:

  NP_001075948.1

• Accession Number mRNA:

  NM_001082479.1

• Reactivity:

  Rat

• Cross Reactivity:

  Rat

• Sequence:

  MGPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA

• Assay Protocol:

  It is recommended to reconstitute the lyophilized rIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  >98.0% as determined by SDS-PAGE

• Bioactivity:

  The ED50 as determined by the stimulation of protein synthesis in rat L6 myoblasts was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg.

• Length:

  71

• Form:

  Lyophilized

• Reconstitution:

  0.4% NaHCO3

• Molecular Weight:

  7.6 kDa

• Host or Source:

  E. coli