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IGF-1

CAT: 0209-500-024Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-500-024Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rabbit IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton.
Synonyms
Igf1; Igf-1; Igf-I; C730016P09Rik
NCBI Gene ID
100008668
UniProt
Q95222
Accession Number
NP_001075495.1
Accession Number mRNA
NM_001082026.1
Reactivity
Rabbit
Cross Reactivity
Rabbit
Sequence
MTGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKAA
Assay Protocol
It is recommended to reconstitute the lyophilized rbIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
RbIGF-I is biologically active when compared to human IGF-1. The ED50, calculated by the dose -dependent proliferation of human MCF/7 cells is 5 to 25 ng/ml in the cell culture mixture dependent on culture conditions. Its activity consisits of 30-40 % compared to human IGF-1.
Length
72
Form
Lyophilized
Reconstitution
0.4% NaHCO3
Molecular Weight
7.6 kDa
Host or Source
E. coli

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