IFN beta Recombinant Protein

• Description:

  Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-alpha is an acid stable molecule that signals through IFN-alpha/betaR, which is also used by IFN-beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFN-beta is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-beta has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

• Synonyms:

  IFNB1; IFB; IFF; IFNB

• CAS Number:

  9000-83-3

• NCBI Gene ID:

  3456

• UniProt:

  P01574

• Accession Number:

  NP_002167.1

• Accession Number mRNA:

  NM_002176

• Gene Location:

  9p21

• Host:

  CHO

• Origin Species:

  Human

• Species Reactivity:

  Monkey, Mouse, Human

• Sequence:

  MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

• Detection Range:

  Assay #1: Determined by its ability to induce STAT1/STAT2 activation in human COLO 205 ISRE LUC reporter cells. Assay #2: Determined by a cytotoxicity assay using human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml (specific activity of ≥ 1 x 10^7 units/mg).

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Length:

  166

• Form:

  Lyophilized

• Molecular Weight:

  20 kDa

• Shipping Conditions:

  Room Temperature