HVEM-Fc Recombinant Protein

HVEM-Fc Recombinant Protein

• Description: HVEM belongs to the TNF Receptor superfamily of transmembrane proteins and plays a role in the activation of T-cells and other lymphocytes. It is expressed in various cells and tissues including spleen, thymus, lung, macrophages, and T-cells. HVEM activation induces a signaling cascade which results in induction of transcription factors NF-kappaB and AP-1. LIGHT (TNFSF14) and TNF-beta (TNFSF1) function as the ligands for HVEM, which can also bind specifically to herpes simplex virus glycoprotein D. Soluble HVEM can act as a “receptor decoy” resulting in inhibition of the activity of the HVEM ligands, LIGHT and TNF-beta. Recombinant human HVEM-Fc Chimera is a 376 amino acid fusion protein containing an N-terminal domain corresponding to the extracellular region of HVEM and a C-terminal domain corresponding to residues 102 to 330 of human IgG1.
• Synonyms: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• CAS Number: 9000-83-3
• NCBI Gene ID: 8764
• UniProt: Q92956
• Accession Number: NP_003811.2
• Accession Number mRNA: NM_003820.2
• Gene Location: 1p36.32
• Host: Insect Cells
• Origin Species: Human
• Species Reactivity: Mouse
• Tag: Fc-tag
• Sequence: LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGTLEE CQHQTKRSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
• Detection Range: Determined by its ability to neutralize 0.25 ng/ml of hTNFβ induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 μg/ml of HVEM-Fc.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 376
• Form: Lyophilized
• Molecular Weight: 41.4 kDa
• Shipping Conditions: Room Temperature