Growth Hormone

• Description:

  Recombinant rat GH-22K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton.

• Synonyms:

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• NCBI Gene ID:

  24391

• UniProt:

  P01244

• Accession Number:

  NP_001030020.2

• Accession Number mRNA:

  NM_001034848.2

• Reactivity:

  Rat

• Sequence:

  AAFPAMPLSSLFANAVLRAQHLHQLAADTYKEFERAYIPEGQRYSIQNAQAAFCFSETIPAPTGKEEAQQRTDMELLRFSLLLIQSWLGPVQFLSRIFTNSLMFGTSDRVYEKLKDLEEGIQALMQELEDGSPRIGQILKQTYDKFDANMRSDDALLKNYGLLSCFKKDLHKAETYLRVMKCRRFAESSCAF

• Assay Protocol:

  It is recommended to reconstitute the lyophilized rGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  RGH is fully biologically active when compared to WHO reference standard using in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .

• Length:

  192

• Form:

  Lyophilized

• Reconstitution:

  Water, pH 9

• Molecular Weight:

  22.3 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AFPAM