Growth Hormone

• Description :

  Recombinant rat GH-22K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton.

• Synonyms :

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• NCBI Gene ID :

  24391

• UniProt :

  P01244

• Accession Number :

  NP_001030020.2

• Accession Number mRNA :

  NM_001034848.2

• Reactivity :

  Rat

• Sequence :

  AAFPAMPLSSLFANAVLRAQHLHQLAADTYKEFERAYIPEGQRYSIQNAQAAFCFSETIPAPTGKEEAQQRTDMELLRFSLLLIQSWLGPVQFLSRIFTNSLMFGTSDRVYEKLKDLEEGIQALMQELEDGSPRIGQILKQTYDKFDANMRSDDALLKNYGLLSCFKKDLHKAETYLRVMKCRRFAESSCAF

• Assay Protocol :

  It is recommended to reconstitute the lyophilized rGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity :

  RGH is fully biologically active when compared to WHO reference standard using in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .

• Length :

  192

• Form :

  Lyophilized

• Reconstitution :

  Water, pH 9

• Molecular Weight :

  22.3 kDa

• Host or Source :

  E. coli

• N Terminal Sequence :

  AFPAM