Growth Hormone

• Description:

  Recombinant ovine growth hormone, one polypeptide chain containing 191 amino acids and having a molecular mass of ~ 21.8 kDa.

• Synonyms:

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• NCBI Gene ID:

  443329

• UniProt:

  P67930

• Accession Number:

  NP_001009315.2

• Accession Number mRNA:

  NM_001009315.3

• Reactivity:

  Ovine

• Sequence:

  AFPAMSLSGLFANAVLRAQHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETIPAPTGKNEAQQKSDLELLRISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDVTPRAGQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF

• Assay Protocol:

  It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

• Bioactivity:

  OGH is fully biologically active when compared to WHO reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .

• Length:

  191

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  21.8 kDA

• Host or Source:

  E. coli

• N Terminal Sequence:

  ATFPA