Growth Hormone

CAT: 0209-500-012SSize: 20 µgDry Ice: NoHazardous: No
CAT#:0209-500-012SSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant ovine growth hormone, one polypeptide chain containing 191 amino acids and having a molecular mass of ~ 21.8 kDa.
Synonyms
Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
NCBI Gene ID
443329
UniProt
P67930
Accession Number
NP_001009315.2
Accession Number mRNA
NM_001009315.3
Reactivity
Ovine
Sequence
AFPAMSLSGLFANAVLRAQHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETIPAPTGKNEAQQKSDLELLRISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDVTPRAGQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF
Assay Protocol
It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Bioactivity
OGH is fully biologically active when compared to WHO reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .
Length
191
Form
Lyophilized
Reconstitution
Water
Molecular Weight
21.8 kDA
Host or Source
E. coli
N Terminal Sequence
ATFPA

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