Granzyme B

• Description:

  Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

• Synonyms:

  Gzmb; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11

• NCBI Gene ID:

  14939

• UniProt:

  Q3TZH4

• Accession Number:

  NP_038570.1

• Accession Number mRNA:

  NM_013542.2

• Chromosomal Location:

  14 D3; 14 20.5 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse protein Granzyme B

• Clone:

  (#14G13)

• Applications:

  WB

• Purification Method:

  Protein G/A chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2