Galectin-1

• Description:

  Galectin-1, also known as L14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting proinflammatory cytokine production. Galectin1 can also modulate cel-lcell as well as cell-lmatrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and antiinflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin1 share about 88% amino acid sequence similarity.

• Synonyms:

  LGALS1; GBP; GAL1

• NCBI Gene ID:

  3956

• UniProt:

  P09382

• Accession Number:

  NP_002296.1

• Accession Number mRNA:

  NM_002305.3

• Chromosomal Location:

  22q13.1

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human Galectin-1 (RT #100-175)

• Clone:

  Rabbit IgG

• Applications:

  WB

• Purification Method:

  Protein A purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: Use 1-5 µg/ml

• Form:

  Lyophilized

• Buffer:

  3 mM PBS, pH 7.2

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit