Galectin-1

• Description:

  Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

• Synonyms:

  Galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14 kDa; GBP, GAL1; LGALS1

• NCBI Gene ID:

  3956

• UniProt:

  P09382

• Accession Number:

  NP_002296.1

• Accession Number mRNA:

  NM_002305.3

• Chromosomal Location:

  22q13.1

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Human

• Sequence:

  ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPF QPGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFDLEHHHH HH

• Assay Protocol:

  Centrifuge vial prior to opening. Human Galectin-1 should be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

• Purity:

  > 95% by SDS-PAGE & visualized by Coomassie stain

• Bioactivity:

  Testing in Progress.

• Length:

  142

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  14,5 kDa

• Storage Conditions:

  The lyophilized human Galectin-1, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Galectin-1 should be stored in working aliquots at -20°C.

• Host or Source:

  E. coli

• N Terminal Sequence:

  ACGLVASNLNLKPGE