FRP-3 (secreted)

• Description:

  Secreted Frizzled Related Protein‑3 (sFRP‑3), also known as FRZB, belongs to a family of Wnt-binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain. Mature human sFRP‑3 shares 93% aa sequence identity with mouse and rat sFRP‑3. It neutralizes the bioactivity of Wnt‑1, ‑5a, ‑9a, Xenopus Xwnt‑8, and EGF (5‑8) . sFRP‑3 regulates somitic myogenesis and osteoblast differentiation as well as morphogenesis of the cochlear epithelium, hair follicle, and cardiac atrioventricular cushion. sFRP‑3 also functions as a tumor suppressor in a variety of cancers including melanoma, medulloblastoma, soft tissue sarcoma, and androgen‑independent prostate cancer. It inhibits epithelial-mesenchymal transition, tumor growth and invasion, and the activity of MMP‑2 and MMP‑9. In contrast, it can promote tumor growth and angiogenesis in renal cancer.

• Synonyms:

  Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1

• NCBI Gene ID:

  2487

• UniProt:

  Q92765

• Accession Number:

  NP_001454.2

• Accession Number mRNA:

  NM_001463.3

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human recombinant sFRP-3

• Clone:

  (#9J18)

• Applications:

  WB

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1