Rat Anti-Mouse EphA7 Monoclonal Antibody

Rat Anti-Mouse EphA7 Monoclonal Antibody

• Description: EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11, is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA7 has been shown to bind ephrinA2, ephrinA3, ephrinA1, ephrinA4, and ephrinA5. The extracellular domains of human and mouse EphA7 share greater than 97% amino acid identity. Only membranebound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis.
• Synonyms: Epha7; Ebk; Ehk3; Mdk1; Cek11; Hek11
• CAS Number: 9007-83-4
• NCBI Gene ID: 13841
• UniProt: Q61772
• Accession Number: NP_034271.3
• Accession Number mRNA: NM_010141.3
• Gene Location: 4 A4; 4 1.9 cM
• Host: Rat
• Origin Species: Mouse
• Species Reactivity: Mouse
• Target Antigen: Recombinant Mouse EphA7 extracellular domain
• Isotype: IgG1
• Clone: (#6E1)
• Validated Applications: WB, IHC (P)
• Purification Method: Protein G/A chromatography
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.