Endothelin-1/ET-1 Recombinant Protein

Endothelin-1/ET-1 Recombinant Protein

• Description: Endothelin-1 (ET-1) is a potent endogenous vasoconstrictor, mainly secreted by endothelial cells. ET-1 acts through two types of receptors: ETA and ETB. Apart from a vasoconstrictive action, ET-1 causes fibrosis of the vascular cells and stimulates production of reactive oxygen species. It is claimed that ET-1 induces proinflammatory mechanisms, increasing superoxide anion production and cytokine secretion. A recent study has shown that ET-1 is involved in the activation of transcription factors such as NF-kappaB and expression of proinflammatory cytokines including TNF-alpha, IL-1, and IL-6. It has been also indicated that during endotoxaemia, the plasma level of ET-1 is increased in various animal species. Some authors indicate a clear correlation between endothelin plasma level and morbidity/mortality rate in septic patients. These pathological effects of ET-1 may be abrogated at least partly by endothelin receptor blockade. ET-1 receptor antagonists may be useful for prevention of various vascular diseases.
• Synonyms: Preproendothelin-1, PPET-1, ET-1, EDN1
• CAS Number: 9000-83-3
• NCBI Gene ID: 1906
• UniProt: P05305
• Accession Number: NP_001946.3
• Accession Number mRNA: NM_01955.4
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: Recombinant Human Endothelin-2
• Tag: His-Tag
• Sequence: MAPETAVLGA ELSAVGENGG EKPTPSPPWR LRRSKRCSCS SLMDKECVYF CHLDIIWVNT PEHVVPYGLG SPRSKRALEN LLPTKATDRE NRCQCASQKD KKCWNFCQAG KELRAEDIME KDWNNHKKGK DCSKLGKKCI YQQLVRGRKI RRSSEEHLRQ TRSETMRNSV KSSFHDPKLK GKPSRERYVT HNRAHWLEHH HHHH
• Purity: > 98% by SDS-PAGE & visualized Coomassie stain
• Length: 204
• Form: Lyophilized from PBS
• Buffer: PBS
• Molecular Weight: 26.0 kDa
• Shipping Conditions: Room Temperature