CCM-1 Recombinant Protein
CAT:
209-300-054
Size:
20 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
CCM-1 Recombinant Protein
- Description: Cerebral cavernous malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.
- Synonyms: CCM-1; Cerebral cavernous malformations protein 1; KRIT1; KRIT1, ankyrin repeat containing; CAM
- CAS Number: 9000-83-3
- NCBI Gene ID: 889
- UniProt: O00522
- Accession Number: NP_004903.2
- Accession Number mRNA: NM_004912.3
- Gene Location: 7q21.2
- Host: E. coli
- Origin Species: Human
- Species Reactivity: Human
- Tag: His-Tag
- Sequence: MLLKEAINKPYEKVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHVRDWPEILAELTNLDPQRETPQLFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQIVYGNYESKKHKQGFLNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLSTSEGVSKEMHHLQRMFLQNCWEIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYGCFMWQLGDTDTCFQIHSMENKMSFIVHTKQAGLVVKLLMKLNGQLMPTERNSLEHHHHH
- Detection Range: Data not available.
- Purity: > 90% by SDS-PAGE
- Length: 335
- Form: Lyophilized
- Buffer: 30mM NaCl. 50 mM NaP, pH 7.4
- Reconstitution: Human CCM1 should be reconstituted in water or other buffer solutions and stored at -20°C.
- Molecular Weight: 39 kDa
- Shipping Conditions: Room Temperature
- Storage Conditions: The lyophilized human CCM1, though stable at room temperature, is best stored desiccated below 0°C.