Rat Anti-Mouse CCL-19 Monoclonal Antibody

Rat Anti-Mouse CCL-19 Monoclonal Antibody

• Description: CCL19/MIP3β, also known as ELC (EBI1Ligand Chemokine), is a β chemokine that binds specifically to the chemokine receptor CCR7/EBI1/ BLR2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other β chemokines (20-30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is downregulated by the antiinflammatory cytokine IL10. Recombinant CCL19 has been shown to be chemotactic for Tcells and Bcells. The CCL19 receptor (CCR7/ EBI1/ BLR2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly upregulated in Bcells infected with EpsteinBarr virus and T-cells infected with herpesvirus 6 or 7.
• Synonyms: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• CAS Number: 9007-83-4
• NCBI Gene ID: 24047
• UniProt: O70460
• Accession Number: NP_036018
• Accession Number mRNA: NM_011888
• Gene Location: 4 A5; 4
• Host: Rat
• Origin Species: Mouse
• Species Reactivity: Mouse
• Target Antigen: Recombinant Mouse CCL-19
• Isotype: IgG2
• Clone: (#10J38)
• Validated Applications: WB, N
• Purification Method: Protein G/A chromatography
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.