BAFF

• Description :

  BAFF, a member of the TNF family of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain. Recombinant human soluble BAFF is a 153 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.

• Synonyms :

  TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20

• NCBI Gene ID :

  10673

• UniProt :

  Q9Y275

• Accession Number :

  NP_006564.1

• Accession Number mRNA :

  NM_006573.4

• Chromosomal Location :

  13q32-q34

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Sequence :

  AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Determined by its dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10 ng/ml.

• Length :

  153

• Form :

  Lyophilized

• Buffer :

  0.5 x PBS pH 7.5 + 0.5 mM DTT

• Molecular Weight :

  17.0 kDa

• Host or Source :

  E. coli