Artemin

Artemin is a member of the Glia Cel-lDerived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβ family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxyterminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands. Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFRα1GFRα4) and a common signaling component, cRET (receptor tyrosine kinase) . Artemin prefers to bind to GFRα3 and activites the GFRα3RET. However, in the presence of RET, it can bind to GFRα1 as well. Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.

Artn; neublastin

11876

Q9Z0L2

NP_033841.1

NM_009711.3

4; 4 D1

Anti-Mouse

Mouse

Recombinant mouse Artemin

(#6D32)

WB, IHC

Protein G chromatography

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Lyophilized

PBS

PBS

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Rat