Amphiregulin (98aa)

• Description:

  Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

• Synonyms:

  AREG; AR; SDGF; AREGB; CRDGF

• NCBI Gene ID:

  374

• UniProt:

  P15514

• Accession Number:

  NP_001648.1

• Accession Number mRNA:

  NM_001657.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 97% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

• Length:

  98

• Form:

  Lyophilized

• Molecular Weight:

  11.3 kDa

• Host or Source:

  E. coli