Proteinase K Recombinant Protein

• Abbreviation:

  Recombinant Tritirachium album proteinase K protein

• Gene Name:

  PROK

• UniProt:

  P06873

• Organism:

  Tritirachium album (Engyodontium album)

• Type:

  Active Protein & In Stock Protein

• Source:

  Yeast

• Field of Research:

  Others

• Endotoxin:

  Not test

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  ①Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated > 30U/mg. Unit definition:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C ②Detect Nucleic acid residue by agarose gel electrophores. ③Detect DNase residue by agarose gel electrophores. ④Detect RNase residue by agarose gel electrophores. ⑤The Proteinase K powder was stored at 4°C. And its enzyme activity was assayed every month over the past year. The left chart shows how its enzyme activity changes in a year. It turns out the enzyme activity is maintained more than 95%, which indicates that proteinase K is very stable.

• Form:

  Liquid or Lyophilized powder

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  28.9 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.