Recombinant murine Interleukin-1 b (rm IL-1b )

• Synonyms:

  Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor , LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP

• Source:

  Escherichia coli.

• Purity:

  > 96 % by SDS-PAGE and HPLC analyses.

• Bioactivity:

  Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 108 IU/mg.

• Molecular Weight:

  Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

• Storage Conditions:

  This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.

• CAS Number:

  9000-83-3