Amyloid Beta 1-42 Oligomers

• Background:

  Our Amyloid Beta 1-42 (Aβ42) Oligomers are generated from Amyloid Beta Peptide 1-42 pre-treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) as previously published (1,2) . Our Aβ42 oligomers present as globular oligomers when observed under TEM and AFM, and have a unique dimer/trimer and oligomer signal on a Western Blot with an anti-amyloid beta antibody. Our Aβ42 oligomers were also demonstrated to be toxic to primary rat cortical neurons in a dose-dependent manner. In the brain, amyloid beta peptide (Aβ) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of Aβ plaques in the brain is considered a hallmark of Alzheimer’s disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (3) . Soluble Aβ oligomers isolated from the brains of AD patients or those generated in vitro potently impaired synapse structure and function (4) . Aβ oligomers generated in vitro were toxic to PC12 cells (5) and SH-SY5Y cells (6) . Aβ was demonstrated to interact with tauopathies to affect neurodegeneration in AD patients (7) and accumulations of Aβ were shown to be associated with lower survival rates in Parkinson’s disease patients with dementia (8) .

• Description:

  Human Synthetic Amyloid Beta 1-42 Oligomers

• Product Name Alternative:

  Abeta Oligomers, Abeta peptide, Amyloid beta peptide oligomers, Beta amyloid peptide oligomers, amyloid beta precursor protein peptide oligomers, APP

• UNSPSC:

  12352202

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  351

• Swiss Prot:

  P05067

• Cellular Locus:

  Cell Membrane | Intracellular Vesicles

• Expression System:

  Synthetic

• Origin Species:

  Human

• Target:

  Amyloid Beta Oligomers

• Conjugation:

  No Tag

• Nature:

  Synthetic (TFA preparation)

• Sequence:

  DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

• Applications:

  WB | In vivo Assay | In vitro Assay

• Field of Research:

  Neuroscience | Neurodegeneration | Alzheimer's Disease | Amyloid

• Limit Of Detection:

  Certified >95% pure using mass spec and HPLC. Low endotoxin <2.5 EU/mL @ 1mg/mL.

• Concentration:

  1 mg/ml

• Purity:

  >95%

• Weight:

  0.05

• Length:

  42 amino acids

• Buffer:

  Phosphate buffer (PB) pH 7.4 and 10 mM NaCl

• Molecular Weight:

  4.5 kDa

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Stine et al. 2003. JBC. 278(13):11612-22. doi: 10.1074/jbc.M210207200 2. Ahmed et al. 2010. Nature Structural & Molecular Biology. 17(5):561-7. doi: 10.1038/nsmb.1799 3. Panza et al. 2019. Nat Rev Neurol. 15:73-88 https://doi.org/10.1038/s41582-018-0116-6 4. Shankar et al. 2008. Nat Med. 14(8):837-842. doi: 10.1038/nm1782 5. Chromy et al. 2003. Biochemistry. 42:12749-12760. doi: 10.1021/bi030029q 6. Kayed et al. 2003. Science. 300(5618): 486-489. doi: 10.1126/science.1079469 7. Want et al. 2016. JAMA Neurol. 73(9):1070-7. doi: 10.1001/jamaneurol.2016.2078 8. Kotzbauer et al. 2012. Arch Neurol. 69(10): 1326-1331. doi: 10.1001/archneurol.2012.1611

• Shipping Conditions:

  Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

• Storage Conditions:

  -80ºC

• Protein Length:

  42 amino acids

• Background Reference 01:

  1. Stine et al. 2003. JBC. 278 (13) :11612-22. doi: 10.1074/jbc.M210207200 2. Ahmed et al. 2010. Nature Structural & Molecular Biology. 17 (5) :561-7. doi: 10.1038/nsmb.1799 3. Panza et al. 2019. Nat Rev Neurol. 15:73-88 https://doi.org/10.1038/s41582-018-0116-6 4. Shankar et al. 2008. Nat Med. 14 (8) :837-842. doi: 10.1038/nm1782 5. Chromy et al. 2003. Biochemistry. 42:12749-12760. doi: 10.1021/bi030029q 6. Kayed et al. 2003. Science. 300 (5618) : 486-489. doi: 10.1126/science.1079469 7. Want et al. 2016. JAMA Neurol. 73 (9) :1070-7. doi: 10.1001/jamaneurol.2016.2078 8. Kotzbauer et al. 2012. Arch Neurol. 69 (10) : 1326-1331. doi: 10.1001/archneurol.2012.1611

• Location:

  Cell Membrane | Intracellular Vesicles

• AA Sequence:

  DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

• Immunogen Species:

  Human