Recombinant Mouse Heme-binding protein 1 (Hebp1)

Recombinant Mouse Heme-binding protein 1 (Hebp1)

• CAS Number: 9000-83-3
• Gene Name: Hebp1
• UniProt: Q9R257
• Expression Region: 1-190aa
• Organism: Mus musculus
• Target Sequence: MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGTNDKGVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESVKIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYGRRNEVWLVKA
• Tag: C-terminal 6xHis-tagged
• Source: Baculovirus
• Field of Research: Cardiovascular
• Assay Type: Developed Protein
• Relevance: May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N-methylprotoporphyrin with similar affinities.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 22.1 kDa
• References & Citations: "The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP." Dias J.S., Macedo A.L., Ferreira G.C., Peterson F.C., Volkman B.F., Goodfellow B.J. J. Biol. Chem. 281:31553-31561 (2006)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.