Anti-CD34(QBEnd/10 + HPCA1/763), CF740 conjugate

Anti-CD34(QBEnd/10 + HPCA1/763), CF740 conjugate

• Description: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD34
• Gene ID: 947
• NCBI Gene ID: 374990
• UniProt: P28906
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10); Recombinant full-length human HPCA1 protein (HPCA1/763)
• Target Antigen: CD34
• Clonality: Monoclonal
• Isotype: IgG
• Clone: QBEnd/10 HPCA1/763
• Conjugation: CF740
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Angiogenesis, Cancer
• Positive Control: KG-1 cells, Tonsil, or Angiosarcoma
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 90-110 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years