Anti-Interferon alpha-2 (IFNA2) (N27), CF740 conjugate

Anti-Interferon alpha-2 (IFNA2) (N27), CF740 conjugate

• Description: This antibody recognizes a protein of 16-27 kDa, identified as human interferon-alphaII (IFN-alphaII). Its epitope maps between aa43-53 of the IFN-alphaII) (total aa172). This MAb is specific for IFN-alpha(II) and does not cross-react with IFN-alpha(I). Epitopes of N27 and N39 MAb s are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-alpha(II) after viral infections. The IFN-alpha family consists of 24 or more genes or pseudo-genes. IFN-alphaII) is one of the two distinct families (I and II) of human IFN-alpha. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Alpha 2a interferon; IFN alpha; IFN-alpha-2; IFNA; IFNA2; IFNA2B; Interferon alpha 2a; Interferon alpha 2b; Interferon alpha-2; Interferon alpha-A; LeIF2; LeIFA
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IFNA2
• Gene ID: 3440 (Human)
• NCBI Gene ID: 211575 (Human)
• UniProt: P01563 (Human)
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified recombinant human IFN-alpha2
• Target Antigen: Interferon Alpha-2
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: N27
• Conjugation: CF740
• Source: Animal
• Applications: Functional studies (published) | ELISA (published) | WB (published)
• Validated Applications: ELISA, WB
• Field of Research: Cytokines, Immunology, Inflammation
• Positive Control: Stimulated human peripheral blood mononuclear cells
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 16-27 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells in 0.1mL|Immunofluorescence: 0.5-1 ug/mL|Blocking activity order Ab without azide|ELISA For coating, order Ab without BSA|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Interferon Cytokine Res (2006) 26:866-876. (ELISA, WB) Immunol Lett (1993) 35(3):281-284. (epitope mapping)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years