Anti-CD3e(RIV9), CF740 conjugate

Anti-CD3e(RIV9), CF740 conjugate

• Description: Reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa.  CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD3 epsilon | CD3 TCR complex | CD3E | CD3e antigen epsilon polypeptide (TiT3 complex) | Leu-4 epsilon chain | T cell antigen receptor complex epsilon subunit of T3 | T-cell surface antigen T3 | T-cell surface glycoprotein CD3 epsilon chain | T3E | TCRE | TiT3 complex
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD3E
• Gene ID: 916
• NCBI Gene ID: 3003
• UniProt: P07766
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Human peripheral lymphocytes
• Target Antigen: CD3
• Clonality: Monoclonal
• Isotype: IgG3 κ
• Clone: RIV9
• Conjugation: CF740
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published) | IHC, frozen (expected) | Flow, surface (verified) | IF (verified) | IP (published)
• Validated Applications: IHC, FC, IF, IP
• Field of Research: Immunology
• Positive Control: Jurkat cells. Tonsil and lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 20 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Transplant Proc (1989) 21: 1026-7. (functional studies) Eur J Immunol (1990) 20(4): 943-945. (immunoprecipitation) Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989. Immunology (1994) 83: 457-464. (immunofluorescence, frozen tissue) Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years