Anti-CD7(B-F12), CF740 conjugate

Anti-CD7(B-F12), CF740 conjugate

• Description: Recognizes a protein of 40 kDa, identified as CD7 (also known as gp40, Leu9). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: GP40; Leu9; p41; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD7
• Gene ID: 924
• NCBI Gene ID: 186820
• UniProt: P09564
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Guinea pig, Human, Pig
• Immunogen: Chronic Lymphoid Leukemia cells (CLL)
• Target Antigen: CD7
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: B-F12
• Conjugation: CF740
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published)
• Validated Applications: Functional assays
• Field of Research: Immunology, Signal transduction
• Positive Control: Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 40 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Bone Marrow Transplantation (1992) 9(5):319-323. (cell depletion)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years