Anti-Complement C4d(C4D204), CF740 conjugate

Anti-Complement C4d(C4D204), CF740 conjugate

• Description: This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Acidic Complement C4; Basic C4; C4-1; C4a Anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4b; C4B1; C4B12; C4d fragment; C4F; C4S; Complement C4 Gamma Chain; Complement Component 4A; Complement Component 4B
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: C4A
• Gene ID: 720 & 721
• NCBI Gene ID: 534847; 720022
• UniProt: P0C0L4; P0C0L5
• Cellular Locus: Secreted (extracellular)|Vesicular
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human Complement 4d protein
• Target Antigen: Complement C4d
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: C4D204
• Conjugation: CF740
• Source: Animal
• Applications: IF (published) | IHC, FFPE (verified)
• Validated Applications: IF, IHC, FFPE
• Field of Research: Inflammation
• Positive Control: Rejected Renal Transplant Tissue
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 192 kDa (predicted)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 1:200-1:400|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 1:50-1:100|ELISA For coating use Ab at 1-2 ug/mL, order Ab without BSA|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Sci Rep (2018) 8: 7348. (IF, FFPE)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years