Anti-CD36(185-1G2), CF740 conjugate

Anti-CD36(185-1G2), CF740 conjugate

• Description: Recognizes a protein of 80 kDa-90 kDa, identified as CD36 (Workshop IV; Code P-26). Its epitope maps between aa155-183. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36. This MAb blocks adhesion of P. falciparum parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Adipocyte membrane protein; CHDS7; Collagen receptor, platelet; fatty acid translocase (FAT); Fatty acid transport protein; glycoprotein iiib (GP IIIb); PAS IV; PAS-4; pas4 protein; platelet collagen receptor; Platelet glycoprotein 4; scarb3; Scavenger receptor class B member 3; thrombospondin receptor
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD36
• Gene ID: 948
• NCBI Gene ID: 120949
• UniProt: P16671
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Stimulated human leukocytes
• Target Antigen: CD36
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: 185-1G2
• Conjugation: CF740
• Source: Animal
• Applications: Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)
• Validated Applications: FC, IF, IP, Functional assays
• Field of Research: Immunology, Inflammation
• Positive Control: HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 80-90 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Blood (2001) 97(6), 1689-1696. (IP) J Biol Chem (2006) 281 (36), 26069-26080. (receptor blockade) J Leukocyte Biol (2014) 95, 509-520. (surface Flow, IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years