Anti-Hu CD41/CD61 (PAC-1 epitope) Alexa Fluor® 488

• Description:

  CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.

• Certification:

  RUO

• Immunogen:

  Human platelets

• Target Antigen:

  CD41/CD61 (PAC-1 epitope)

• Clone:

  PAC-1

• Applications:

  ICC, FC (QC tested)

• Format:

  Alexa Fluor® 488

• Buffer:

  Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• References & Citations:

  *Shattil SJ, Hoxie JA, Cunningham M, Brass LF: Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. J Biol Chem. 1985 Sep 15;260 (20) :11107-14. , URL: http://www.ncbi.nlm.nih.gov/pubmed/2411729, *Shattil SJ, Cunningham M, Hoxie JA: Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 1987 Jul;70 (1) :307-15. , URL: http://www.ncbi.nlm.nih.gov/pubmed/3297204 , *Trikha M, Timar J, Lundy SK, Szekeres K, Cai Y, Porter AT, Honn KV: The high affinity alphaIIb beta3 integrin is involved in invasion of human melanoma cells. Cancer Res. 1997 Jun 15;57 (12) :2522-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/9192835 , *Bourne JH, Colicchia M, Di Y, Martin E, Slater A, Roumenina LT, Dimitrov JD, Watson SP, Rayes J: Heme induces human and mouse platelet activation through C-type-lectin-like receptor-2. Haematologica. 2021 Feb 1;106 (2) :626-629., URL: https://pubmed.ncbi.nlm.nih.gov/32354867/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgM kappa