Anti-Hu CD15 Purified

Anti-Hu CD15 Purified

• Background: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
• Specifications: Mouse monoclonal antibody W6D3 recognizes CD15 in nonterminal position on extracellular glycoproteins. CD15 (a cell membrane 3-fucosyl-N-acetyllactosamine 3-FAL) is strongly expressed on granulocytes, monocytes, macrophages, mast cells it is also present on Langerhans cells and some myeloid precursors cells. This is a non-IgM anti-CD15 antibody.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Target Antigen: CD15
• Isotype: Igg1 κ
• Clone: W6D3
• Applications: Flow cytometry: Recommended dilution: 1-4 µg/mL
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Mandalasi M, Dorabawila N, Smith DF, Heimburg-Molinaro J, Cummings RD, Nyame AK: Development and characterization of a specific IgG monoclonal antibody toward the Lewis x antigen using splenocytes of Schistosoma mansoni-infected mice. Glycobiology. 2013 Jul23(7):877-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/23542315,*Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul94(4):637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839,*Brazil JC, Sumagin R, Cummings RD, Louis NA: Targeting of neutrophil Lewis X blocks transepithelial migration and increases phagocytosis and degranulation. Am J Pathol. 2016 Feb186(2):297-311., URL: https://pubmed.ncbi.nlm.nih.gov/26687991/,*Carrel J, Groves CJ: OMIP-043: Identification of human antibody secreting cell subsets. Cytometry A. 2018 Feb93(2):190-193., URL: https://pubmed.ncbi.nlm.nih.gov/29286577/,
• Other References: *Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep39(1-2):185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/,*Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov24(11):771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/,*Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1406(2):333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/,
• Storage Conditions: Store at 2-8°C. Do not freeze.