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Mouse IgG3 Kappa (κ) isotype Control

CAT: 0247-OPRA03551-1MGSize: 1 mgDry Ice: NoHazardous: No
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CAT#:0247-OPRA03551-1MGSize:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reactivity
Mouse
Target
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda._x005F
Clonality
Monoclonal
Conjugation
Unconjugated
Type
Protein
Applications
Enzyme-linked immunosorbent assay|Flow cytometry|Fluorescence-linked immunosorbent assay|Immunofluorescence|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Purification
Mouse IgG3 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.953 mg/ml
Format
Liquid (sterile filtered)
Reconstitution
2°C to 8°C|-20°C
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition confirmed by ELISA.
Protein Length
Native
Host or Source
Mouse

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