SRAGE Protein

• Gene Aliases:

  RAGE, sRAGE, SCARJ1

• Gene ID:

  177

• Accession Number:

  NP_001127.1

• Reactivity:

  Human

• Target:

  SRAGE is a member of the immunoglobulin superfamily of cell surface molecules. sRAGE is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. sRAGE lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. sRAGE mediates interactions of nonenzymatic glycosylated proteins which accumulate in vascular tissue during aging & at an increasing rate in diabetes. sRAGE is a receptor for amyloid beta peptide. sRAGE Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids.

• Type:

  Protein

• Purification:

  Proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml (prior to lyophilization)

• Format:

  Lyophilized// 30mM acetate buffer (pH 4)

• Reconstitution:

  Store lyophilized protein at -20° C. Add 0.1M Acetate buffer (pH 4) to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4° C for a limited period of time.

• Molecular Weight:

  36.5 kDa

• Notes:

  Formerly GWB-1D1558

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  SRAGE

• Host or Source:

  E. Coli

• Protein Name:

  Advanced glycosylation end product-specific receptor

• Gene Name URL:

  SRAGE