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JEV Protein

CAT: 0247-OPPA01477-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA01477-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
JEV, Japanese Encephalitis Virus ENV Recombinant
Reactivity
Japanese Encephalitis Virus
Target
Japanese encephalitis previously known as Japanese B encephalitis is a virus from the family Flaviviridae. it is closely related to the West Nile virus and St. Louis encephalitis virus. Positive sense single stranded RNA genome is packaged in the capsid, formed by the capsid protein. The outer envelope is formed by envelope (E) protein and is the protective antigen. It aids in entry of the virus to the inside of the cell. The genome also encodes several nonstructural proteins also (NS1, NS2a, NS2b, NS3, N4a, NS4b, NS5) . NS1 is produced as secretory form also. NS3 is a putative helicase, and NS5 is the viral polymerase. It has been noted that the Japanese encephalitis virus (JEV) infects the lumen of the endoplasmic reticulum (ER) and rapidly accumulates substantial amounts of viral proteins for the JEV. Japanese Encephalitis is diagnosed by detection of antibodies in serum and CSF (cerebrospinal fluid) by IgM capture ELISA. The E.coli derived recombinant protein contains full length Japanese Encephalitis virus ENV antigen.
Type
Protein
Applications
ELISA, WB
Purification
Purified by proprietary chromatographic techniques.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// 20 mM Tris-MES (pH 6.5), 8 M urea, 200 mM NaCl & 0.05% Tween-20
Reconstitution
JEV, although stable at 4° C for one week, should be stored below -18° C. Please prevent freeze/thaw cycles.
Molecular Weight
50 kDa
Notes
Formerly GWB-721816
Specificity
Immunoreactive with sera of encephalitis virus infected individuals.
Protein Length
Recombinant
NCBI Gene Symbol
ENCEPHALITIS JAPANESE
Gene Name URL
Encephalitis Japanese

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