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NMM Protein

CAT: 0247-OPPA01279-2UGSize: 2 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA01279-2UGSize:2 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
Non-Muscle Myosin-II Regulatory Light Chain, NMM, NMM Human, Non-Muscle Myosin-II Regulatory Light Chain Human Recombinant
Reactivity
Human
Target
Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca +2 Calmodulin regulated light chain kinase (MLCK) .
Type
Protein
Source
E. coli
Sequence
MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Bioactivity
Activity: //Assayed for phosphorylation by MLCK.
Format
NMM protein in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT. Physical appearance: Sterile Filtered clear solution.
Reconstitution
Store vial at -20C to -80C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.
Notes
Formerly GWB-144D34
Protein Length
Recombinant
NCBI Gene Symbol
NMM
Gene Name URL
NMM

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