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TaqDNA Protein

CAT: 0247-OPPA00828-1000IUSize: 1000 UnitsDry Ice: NoHazardous: No
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CAT#:0247-OPPA00828-1000IUSize:1000 Units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Taq DNA Polymerase (a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74° C and exhibits a half-life of 40 minutes at 95° C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Gene Aliases
DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1, TaqDNA, Taq DNA Polymerase Recombinant
Target
Recombinant Taq DNA Polymerase
Type
Protein
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
20 IU/uL
Bioactivity
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74° C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25° C), 50mM NaCl, 5mM MgCl2, 200 um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.
Format
Liquid// 20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% NP40, 0.5% Tween-20
Reconstitution
Stable for 5 days at 10° C, for longer period of time store at -20° C.
Notes
Formerly GWB-CBD318
NCBI Gene Symbol
TAQDNA
Host or Source
Recombinant e.coli contains Thermus aquaticus polymerase gene
Protein Name
DNA polymerase I, thermostable
Gene Name URL
TaqDNA

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