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Lysostaphin Protein

CAT: 0247-OPPA00791-1MGSize: 1 mgDry Ice: NoHazardous: No
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CAT#:0247-OPPA00791-1MGSize:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase, Lysostaphin, Lysostaphin Recombinant
Target
Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination. Lysostaphin Recombinant produced in E.Coli is a non-glycosylated polypeptide chain.
Type
Protein
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Bioactivity
Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8, 30° C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor. Determined to be 3,540 units/mg.
Format
Lyophilized//without additives
Reconstitution
It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted. Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18° C. Upon reconstitution Lysostaphin can be stored at 4° C up to 3 weeks and for future use below -18° C. Please prevent freeze-thaw cycles.
Molecular Weight
27 kDa
Notes
Formerly GWB-80A991
Protein Length
Recombinant
NCBI Gene Symbol
LYSOSTAPHIN
Host or Source
E. Coli
Protein Name
Lysostaphin
Gene Name URL
Lysostaphin

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Alternative Products

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