Leptin Protein

• Gene Aliases:

  Ob, obese

• Gene ID:

  16846

• Accession Number:

  NP_032519.1

• Reactivity:

  Mouse

• Target:

  A peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Leptin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids.

• Type:

  Protein

• Purification:

  Purified by proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml (prior to lyophilization)

• Bioactivity:

  Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

• Format:

  Lyophilized// from a concentrated solution containing 0.1% TFA. Physical appearance:// Sterile Filtered White powder.Physical appearance:// Sterile Filtered White powder.

• Reconstitution:

  2

• Molecular Weight:

  16 kDa

• Notes:

  Formerly GWB-44A721

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  MLEPTIN

• Host or Source:

  E.coli

• Protein Name:

  Leptin

• Gene Name URL:

  MLeptin