Leptin Protein

• Gene Aliases:

  OB, obese

• Gene ID:

  25608

• Accession Number:

  NP_037208.1

• Reactivity:

  Rat

• Target:

  A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids.

• Type:

  Protein

• Sequence:

  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His.

• Purification:

  Purified by proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Bioactivity:

  Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

• Format:

  Lyophilized// from 0.0045mM NaHCO3. Physical Appearance:// Freeze-dried powder3Physical Appearance:// Freeze-dried powder

• Reconstitution:

  2

• Molecular Weight:

  16 kDa

• Notes:

  Formerly GWB-F9D820

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  RLEPTIN

• Host or Source:

  E. Coli

• Protein Name:

  Leptin

• Gene Name URL:

  RLeptin