BDNF Protein

• Gene Name:

  Brain-derived neurotrophic factor

• Gene Aliases:

  ANON2, BULN2

• Gene ID:

  627

• Accession Number:

  NP_001137277.1

• Reactivity:

  Human

• Target:

  BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. the versatility of bdnf is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

• Type:

  Protein

• Source:

  E.coli

• Purification:

  BDNF Human Recombinant is purified by proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Bioactivity:

  The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2 ug/ml.

• Format:

  Lyophilized// without additives. Physical appearance:// Sterile filtered white powder..Physical appearance:// Sterile filtered white powder..

• Reconstitution:

  It is recommended to reconstitute the lyophilized Brain-derived Neurotrophic Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Brain-derived Neurotrophic Factor should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Molecular Weight:

  27 kDa

• Notes:

  Formerly GWB-7ED615

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  BDNF

• Protein Name:

  Brain-derived neurotrophic factor

• Gene Name URL:

  BDNF