CK-BB Protein

• Gene Aliases:

  Creatine Kinase BB (CK-BB) Isoenzyme Recombinant

• Target:

  Full length Creatine Kinase BB isoenzyme with amino acid sequence identical to the native enzyme. CK-BB is a dimeric protein comprised of 2 identical B subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to BB isoenzyme in ELISA.

• Type:

  Protein

• Source:

  Pichia pastoris

• Applications:

  Enzyme immunoassay

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  ®

• Bioactivity:

  1,922 IU/mg. One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction.

• Format:

  Liquid// 10mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA, 50% glycerol (pH 6.0), with no preservatives

• Reconstitution:

  Centrifuge before opening to ensure complete recovery of vial contents.// _x005F Store at -70°C or below. Aliquot to avoid multiple freeze/thaw cycles.

• Molecular Weight:

  47 kDa

• Protein Length:

  Recombinant