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CK-BB Protein

CAT: 0247-OPMA04783-1MGSize: 1 mgDry Ice: NoHazardous: No
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CAT#:0247-OPMA04783-1MGSize:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
Creatine Kinase BB (CK-BB) Isoenzyme Recombinant
Target
Full length Creatine Kinase BB isoenzyme with amino acid sequence identical to the native enzyme. CK-BB is a dimeric protein comprised of 2 identical B subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to BB isoenzyme in ELISA.
Type
Protein
Source
Pichia pastoris
Applications
Enzyme immunoassay
Purification
Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
®
Bioactivity
1,922 IU/mg. One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction.
Format
Liquid// 10mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA, 50% glycerol (pH 6.0), with no preservatives
Reconstitution
Centrifuge before opening to ensure complete recovery of vial contents.// _x005F Store at -70°C or below. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight
47 kDa
Protein Length
Recombinant

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