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UBE2I Recombinant Protein (Human)

CAT: 0247-OPED00149-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OPED00149-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
Gene Name
Ubiquitin conjugating enzyme E2 I
Gene Aliases
C358B7.1; P18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; UBC9; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) ; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) ; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I.
Gene ID
7329
Reactivity
Homo sapiens|Human
Target
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Type
Protein
Purification
Purified by ion-exchange chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid. In 20mM HEPES, pH 7.3, containing 110mM potassium acetate, 2mM magnesium acetate, 0.8mM EGTA, and 1mM DTT.
Reconstitution
Store at -80C
Molecular Weight
18 kDa
Protein Length
Recombinant
NCBI Gene Symbol
UBE2I
Host or Source
E. Coli
Protein Name
SUMO-conjugating enzyme UBC9
Gene Name URL
UBE2I