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UBA3 Recombinant Protein (Human)

CAT: 0247-OPED00101-50UGSize: 50 µgDry Ice: NoHazardous: No
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CAT#:0247-OPED00101-50UGSize:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
Gene Name
Ubiquitin like modifier activating enzyme 3
Gene Aliases
HUBA3; NAE2; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3, ubiquitin-activating enzyme E1 homolog; UBE1C; ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; ubiquitin-activating enzyme E1C (homologous to yeast UBA3) ; ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) ; Ubiquitin-like modifier-activating enzyme 3.
Gene ID
9039
Accession Number
NP_001239007.1
Reactivity
Homo sapiens|Human
Target
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Type
Protein
Purification
Copurified in His-tagged form with Uba3; copurified with APP-BP1.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid. In 25mM HEPES, pH 7.5, containing 150mM NaCl, 1mM DTT.
Reconstitution
Store at -80C
Molecular Weight
62 kDa, 53 kDa
Protein Length
Recombinant
NCBI Gene Symbol
UBA3
Host or Source
E. Coli
Protein Name
NEDD8-activating enzyme E1 catalytic subunit
Gene Name URL
UBA3
Nucleotide Accession Number
NM_001252078.1

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