HSPB1 Recombinant Protein (Human)

• Description:

  Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alpha B-crystallin. It is characterized by a conserved C-terminal alpha -crystallin domain consisting of two anti-parallel beta -sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha , p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

• Gene Name:

  Heat shock protein family B (small) member 1

• Gene Aliases:

  28 kDa heat shock protein; CMT2F; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; putative heat shock protein family B member 1 isoform; SRP27; stress-responsive protein 27.

• Gene ID:

  3315

• Accession Number:

  NP_001138610.1

• Reactivity:

  Homo sapiens|Human

• Target:

  Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975) . Plays a role in stress resistance and actin organization (PubMed:19166925) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742) .

• Type:

  Protein

• Applications:

  WB

• Purification:

  Purified by multi-step chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  Varies by lot. See vial for concentration.

• Format:

  Liquid// 1X Dulbecco's PBS

• Reconstitution:

  Store at -80° C. Avoid repeated freeze/thaw cycles.

• Molecular Weight:

  27 kDa

• Specificity:

  Immunoreactive with antibody specific for Hsp27

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  HSPB1

• Host or Source:

  E. Coli

• Protein Name:

  Heat shock protein beta-1

• Gene Name URL:

  HSPB1

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_001145138.1