TOP3A Recombinant Protein

• Gene Name :

  DNA topoisomerase III alpha

• Gene Aliases :

  DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; MGRISCE2; PEOB5; TOP3; topo III-alpha; topoisomerase (DNA) III alpha; ZGRF7; zinc finger, GRF-type containing 7.

• Gene ID :

  7156

• Accession Number :

  NP_001307688.1

• Reactivity :

  Homo sapiens|Human

• Target :

  Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

• Conjugation :

  Unconjugated

• Type :

  Protein

• Source :

  E.coli

• Applications :

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  200 ug/mL (prior to lyoph)

• Format :

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution :

  2°C to 8°C|-80°C

• Molecular Weight :

  19kDa

• Protein Length :

  Recombinant

• NCBI Gene Symbol :

  TOP3A

• Host or Source :

  E.coli

• Protein Name :

  DNA topoisomerase 3-alpha

• Gene Name URL :

  TOP3A

• CAS Number :

  9000-83-3

• Nucleotide Accession Number :

  NM_001320759.1