PITRM1 Recombinant Protein

• Gene Name:

  Pitrilysin metallopeptidase 1

• Gene Aliases:

  Metalloprotease 1 (pitrilysin family) ; MP1; pitrilysin metalloproteinase 1; PreP; PreP peptidasome; presequence protease, mitochondrial; SCAR30.

• Gene ID:

  10531

• Accession Number:

  NP_001229236.1

• Reactivity:

  Homo sapiens|Human

• Target:

  Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing (PubMed:10360838, PubMed:16849325, PubMed:19196155, PubMed:24931469) . Has an ATP-independent activity (PubMed:16849325) . Specifically cleaves peptides in the range of 5 to 65 residues (PubMed:19196155) . Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference (PubMed:10360838, PubMed:19196155, PubMed:24931469) . Degrades the transit peptides of mitochondrial proteins after their cleavage (PubMed:19196155) . Also degrades other unstructured peptides (PubMed:19196155) . It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion (PubMed:16849325, PubMed:24931469) . It is a highly efficient protease, at least toward amyloid-beta protein 40 (PubMed:24931469) . Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently (PubMed:24931469) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  30kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  PITRM1

• Host or Source:

  E.coli

• Protein Name:

  Presequence protease, mitochondrial

• Gene Name URL:

  PITRM1

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_001242307.1