GDF5 Recombinant Protein

• Gene Name:

  Growth differentiation factor 5

• Gene Aliases:

  BDA1C; BMP14; BMP-14; bone morphogenetic protein 14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP1; DUPANS; growth/differentiation factor 5; LAP4; LAP-4; lipopolysaccharide-associated protein 4; LPS-associated protein 4; OS5; radotermin; SYM1B; SYNS2.

• Gene ID:

  8200

• Accession Number:

  NP_000548.2

• Reactivity:

  Homo sapiens|Human

• Target:

  Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592) . Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273) . Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity) . Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  18kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  GDF5

• Host or Source:

  E.coli

• Protein Name:

  Growth/differentiation factor 5

• Gene Name URL:

  GDF5

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_000557.4