ESR1 Recombinant Protein

• Gene Name:

  Estrogen receptor 1

• Gene Aliases:

  C-terminally-truncated estrogen receptor alpha product-1; Er alpha; ER-alpha; Esr; estradiol receptor; estrogen receptor; estrogen receptor 1 (alpha) ; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha i45aL; estrogen receptor alpha i45aS; estrogen receptor alpha i45c; estrogen receptor alpha i56a; estrogen receptor alpha i56b; estrogen receptor alpha i56c; estrogen receptor alpha i56dL; estrogen receptor alpha i56dS; estrogen receptor alpha i56e; estrogen receptor alpha variant delta 4; estrogen receptor protein; nuclear receptor subfamily 3 group A member 1; RNESTROR; truncated estrogen receptor product-2; trunctaed estrogen receptor product-1.

• Gene ID:

  24890

• Accession Number:

  NP_036821.1

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (By similarity) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  57kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  Esr1

• Host or Source:

  E.coli

• Protein Name:

  Estrogen receptor

• Gene Name URL:

  Esr1

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_012689.1